Analysis of DNA Markers from Vietnamese Asarum L. Species

The Asarum L. genus includes 128 species with a lot of medicinal effects and has been used extensively in the folk prescriptions for treatment several diseases such as bronchitis, asthma, rheumatism, hepatitis. In world, many researches showed that have kinds essential oils myristicin, methyl eugenol, myrcene, asafrol, borneol, safrol or α-pinene substances secquiterpenes, sterols, naringenin glycosyl flavonoids. Some reports Vietnam conducted on analyzing secondary compounds these species. However, it done little research classification through molecular markers. Indeed, DNA barcoding will help overcome difficulties recognizing plants which were endangered is rare materials high value medicine. study, four regions - ITS1, ITS2, matK rpoC analyzed from some are three A. splendens, yentuense petelotii. barcodes well amplified by PCR sequenced Sanger principal. After that, sequences ITS2 had highest polymorphism, addition, markers marker absolutely suitable distinguishing above results great significance application identification species, order to correctly use plant sources.